利用某些微生物代謝過程中產生酶的特點,篩選出能夠大量生成葡萄糖氧化酶的地衣芽孢桿菌,并將其固定在Sephadex 100 或海藻酸納- 氯化鈣(載體) 上,首次研制成微生物酶傳感器. 它不僅具有酶傳感器的所有優點,同時也克服了酶傳感器的缺點,使用壽命長,性質穩定,成本低,易于保存等. 通過優化微生物和其代謝所產生的葡萄糖氧化酶的固定化方法和利用葡萄糖氧化酶氧化葡萄糖產生過氧化氫的反應機理,建立了模擬酶(hemin) 催化熒光底
物N , N′2 二腈甲基鄰苯二氨(DCM2OPA) 檢測體液中葡萄糖含量的熒光傳感器.
關鍵詞　微生物傳感器,酶傳感器,熒光,葡萄糖
Abstract 　A microbial based biosensor for the measurement of glucose in serum and urine has been developed. Bscillus licheniformis , a microbial , was used in the cytosensor as the producer of glucose oxidase that are required in the biosensor. The influence of the types of microbes , enzyme immobilizing techniques and the carrier for the enzyme or microbial were studied. The new sensor has the advantages of high stability , low cost and easier preservation. It is the first time to develop a cytosensor with a mimic enzyme catalyzed fluorescence reaction for the measurement of glucose in serum and urine.
Keywords 　microbial sensor , enzyme sensor , fluorescence , chemiluminescence , glucose